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CD27 signals through PKC in human B cell lymphomas
1Laboratory of Molecular Immunoregulation, Division of Basic Science, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD, USA.
Cytokine
|July 27, 1999
Summary
Tumour necrosis factor receptor (TNFR) superfamily member CD27 and its ligand CD70 regulate B cell interactions. Cross-linking CD27 in lymphoma cells increased proliferation and PKC activation, offering systems to study CD27 signaling.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Tumor necrosis factor receptor (TNFR) superfamily members are crucial for cell proliferation and death.
- CD27 is a unique, covalently linked homodimer within the TNFR superfamily.
- CD27 and its ligand CD70 are involved in T and B cell interactions, activation, and immunoglobulin regulation.
Purpose of the Study:
- To screen B cell lymphoma cell lines for CD27 and CD70 expression.
- To evaluate CD27 activation through antibody cross-linking.
- To identify cellular systems for studying CD27 signal transduction in B cell lymphomas.
Main Methods:
- Screening of B cell lymphoma cell lines for CD27 and CD70 expression.
- Antibody cross-linking of CD27 to induce activation.
- Assessment of cellular proliferation, PKC activation, and cell surface IgG expression.
Main Results:
- Two cell lines, HT and SU-4, exhibited increased cellular proliferation upon CD27 cross-linking.
- This proliferation correlated with enhanced protein kinase C (PKC) activation.
- In the HT cell line, CD27 cross-linking led to increased cell surface IgG expression.
Conclusions:
- Identified HT and SU-4 cell lines as suitable systems for studying CD27 signal transduction.
- Demonstrated that CD27 activation can promote proliferation and modulate immunoglobulin expression in B cell lymphomas.
- Established a foundation for defining the CD27 signaling cascade in specific B cell lymphomas.