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Molecular cloning, sequence analysis, and characterization of a penicillin-resistant DD-carboxypeptidase of
Y Kimura1, Y Takashima, Y Tokumasu
1Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan. kimura@ag.kagawa-u.ac.jp
Abstract:
We have cloned a gene, pdcA, from the genomic library of Myxococcus xanthus with an oligonucleotide probe representing conserved regions of penicillin-resistant DD-carboxypeptidases. The amino- and carboxy-terminal halves of the predicted pdcA gene product showed significant sequence similarity to N-acetylmuramoyl-L-alanine amidase and penicillin-resistant DD-carboxypeptidase, respectively. The pdcA gene was expressed in Escherichia coli, and the characteristics of the gene product were similar to those of DD-carboxypeptidase (VanY) of vancomycin-resistant enterococci. No apparent changes in cell growth, sporulation, or germination were observed in pdcA deletion mutants.