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Cross talk between melatonin and TGFbeta1 in human benign prostate epithelial cells
A Rimler1, H Matzkin, N Zisapel
1Department of Neurobiochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
The Prostate
|July 27, 1999
Summary
Melatonin suppresses benign prostate epithelial cell growth by increasing transforming growth factor-beta1 (TGFbeta1) release. This mechanism is crucial for melatonin's growth-attenuating effects in the prostate.
Area of Science:
- Endocrinology
- Cell Biology
- Prostate Cancer Research
Background:
- Human benign prostate epithelial cells express melatonin receptors.
- Melatonin transiently suppresses DNA synthesis and attenuates cell growth.
- Transforming growth factor-beta1 (TGFbeta1) is produced by prostate cells and inhibits their growth.
Purpose of the Study:
- To investigate the role of TGFbeta1 in melatonin's action on prostate epithelial cells.
- To determine how melatonin affects TGFbeta1 production and activity.
Main Methods:
- Assessed the effects of melatonin and TGFbeta1 on (3)H-thymidine incorporation.
- Investigated melatonin's potential to induce TGFbeta1 release from cells.
- Utilized TGFbeta1-neutralizing antisera to block melatonin's effects.
Main Results:
- TGFbeta1 dose-dependently inhibited (3)H-thymidine incorporation.
- Melatonin inhibited DNA synthesis, with effects modulated by TGFbeta1 presence.
- Melatonin enhanced protein release, including TGFbeta1-like proteins, and neutralizing antisera blocked melatonin's inhibitory action.
Conclusions:
- TGFbeta1 plays a significant role in mediating melatonin's growth-attenuating effects on benign prostate epithelial cells.
- Melatonin-induced TGFbeta1 release contributes to the observed inhibition of cell growth.