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Elastin gene expression in benign prostatic hyperplasia.

B Djavan1, V Lin, C Seitz

  • 1Department of Urology, University of Vienna, Vienna, Austria. bdjavan@hotmail.com

The Prostate
|July 27, 1999
PubMed
Summary
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Elastin mRNA gene expression is significantly downregulated in benign prostatic hyperplasia (BPH) tissues. This study quantifies elastin mRNA levels in BPH versus normal prostate tissue, revealing a 70% decrease in BPH.

Area of Science:

  • Urology
  • Molecular Biology
  • Biochemistry

Background:

  • Benign prostatic hyperplasia (BPH) involves stromal changes, including altered collagen expression.
  • Elastin gene expression in BPH is poorly understood due to alternative splicing complexities.

Purpose of the Study:

  • To quantify elastin gene expression in benign prostatic hyperplasia (BPH) tissues.
  • To investigate the role of elastin in BPH pathophysiology.

Main Methods:

  • Utilized competitive reverse transcriptase-polymerase chain reaction (RT-PCR) for quantitative elastin mRNA analysis.
  • Employed newly designed elastin primers (BOB-1 and BOB-2) to circumvent alternative splicing issues.
  • Analyzed elastin mRNA concentrations in normal (n=10) and BPH (n=12) prostate tissues.

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Main Results:

  • Developed specific primers that accurately amplified elastin cDNA, avoiding alternative splicing regions.
  • Demonstrated significantly lower elastin mRNA concentration in BPH tissues (53 attomole/mg) compared to normal tissues (140.6 attomole/mg).
  • Observed a 70% downregulation of elastin mRNA in BPH tissues.

Conclusions:

  • Elastin mRNA gene expression is significantly downregulated in the transition zone of BPH patients.
  • The findings suggest a substantial reduction in elastin in BPH, contributing to stromal alterations.