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Enzymatic modification of hydroxyethylcellulose by transgalactosylation with beta-galactosidases
1Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Carbohydrate Research
|July 27, 1999
Abstract:
beta-galactosidases from A. oryzae and a thermophilic CLONEZYME glycosidase library were used to catalyze the transfer of the beta-D-galactopyranosyl moiety from lactose to the hydroxyl groups of hydroxyethylcellulose (HEC) in sodium acetate buffer. The degree of substitution was quantified by using galactose oxidase enzymatic assays. Depolymerization was also observed in the course of the transglycosylation reactions.
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