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[Rapid method of isolation of lipid A from Yersinia pseudotuberculosis]
I N Krasikova1, S I Bakholdina, T F Solov'eva
1Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok, Russia. innakras@piboc.marine.su
Abstract:
The possibility of preparing the lipid A (LA) from Yersinia pseudotuberculosis Serovar IB by the hydrolysis of whole cells instead of the preliminary isolation of lipopolysaccharide (LPS) was demonstrated. Direct extraction with an organic solvent of the bacterial mass preliminary treated with 10% acetic acid or 1 M HCl was shown to result in a di- (LAAcOH) or monophosphoryl derivative (LAHCl), respectively. These were completely extractable only after treatment with strong hydrolyzing agents. We concluded that two forms of LA (and LPS) exist in the pseudotuberculosis bacterium which differ in the stability of their bonding to the bacterial outer membrane.