Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Corneal changes in manganese-deficient rats.

H Gong1, T Amemiya

  • 1Department of Ophthalmology, Nagasaki University School of Medicine, Japan. d394047a@stcc.nagasaki-u.ac.jp

Cornea
|July 28, 1999
PubMed
Summary

Dietary manganese (Mn) deficiency significantly alters corneal structure in rats, affecting the epithelium, stroma, and endothelium. Supplementation can restore corneal integrity, highlighting manganese

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research progress on poor response, resistance mechanisms, and influencing factors of immunotherapy for hepatocellular carcinoma].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2026
Same author

[Analysis on HIV infection status and correlated factors in men who have sex with men in young students in Tianjin based on social network peer mobilization testing model].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
Same author

[Clinical and genetic characteristics of X-linked intellectual disability associated with HUWE1 gene variants].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[Research progress on the impact of metabolic associated fatty liver disease on viral activity, treatment response, and prognosis in patients with chronic hepatitis B: current status and prospects].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same author

Resveratrol alleviates stress-associated bursal injury in chickens: a transcriptomic analysis.

British poultry science·2025
Same author

Erratum: Centrality-Dependent Modification of Jet-Production Rates in Deuteron-Gold Collisions at sqrt[s_{NN}]=200  GeV [Phys. Rev. Lett. 116, 122301 (2016)].

Physical review letters·2025

Area of Science:

  • Ophthalmology
  • Nutritional Science
  • Cell Biology

Background:

  • Manganese (Mn) is an essential trace element with poorly understood roles in ocular tissues.
  • Dietary deficiencies can impact cellular structure and function.
  • The cornea's specific dependence on manganese requires further investigation.

Purpose of the Study:

  • To investigate the structural changes in the rat cornea resulting from dietary manganese deficiency.
  • To understand the significance of manganese for corneal health and maintenance.

Main Methods:

  • Wistar-Kyoto rats were fed a manganese-deficient diet across two generations.
  • Corneal tissues were analyzed using transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
  • A recovery group was studied after reintroducing a normal diet post-deficiency.

Main Results:

  • Manganese deficiency led to reduced microvilli, abnormal mitochondria, and altered tonofibrils in the corneal epithelium.
  • The corneal stroma thinned with decreased collagen fiber diameter, and Descemet's membrane was thinner.
  • Scanning electron microscopy revealed fewer epithelial microvilli, more dark cells, and altered endothelial cell shape (pentagonal vs. hexagonal).

Conclusions:

  • Dietary manganese is crucial for maintaining the structural integrity of corneal cells.
  • Corneal structure can largely recover after manganese repletion.
  • This study underscores the importance of manganese in ocular health.

Related Experiment Videos