Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tension modulates cell surface motility: A scanning acoustic microscopy study.

I Karl1, J Bereiter-Hahn

  • 1Cinematic Cell Research Group, Department of Zoology, Biocenter, Johann Wolfgang Goethe University, Frankfurt/Main, Germany.

Cell Motility and the Cytoskeleton
|July 28, 1999
PubMed
Summary

Cell surface tension directly influences cell motility. Increased tension, whether internal or external, reduces cell surface activity, while decreased tension enhances it, revealing a direct relationship.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Keratinocytes and neutrophils are important sources of proinflammatory molecules in hidradenitis suppurativa.

The British journal of dermatology·2015
Same author

High Resolution Strain Analysis Comparing Aorta and Abdominal Aortic Aneurysm with Real Time Three Dimensional Speckle Tracking Ultrasound.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2015
Same author

Advanced 3D-Sonographic Imaging as a Precise Technique to Evaluate Tumor Volume.

Translational oncology·2014
Same author

TRAF2 inhibits TRAIL- and CD95L-induced apoptosis and necroptosis.

Cell death & disease·2014
Same author

[Development of an engraftable skin equivalent based on matriderm with human keratinocytes and fibroblasts].

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2009
Same author

Measuring elastic properties of cells by evaluation of scanning acoustic microscopy V(Z) values using simplex algorithm.

Biophysical journal·2009

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Cell surface motility, including protrusions and ruffling, is crucial for cellular functions.
  • Tension has been theorized as a key regulator of cell shape and surface dynamics.
  • Subcellular motility domains are metabolically dependent and linked to cell surface deformations.

Purpose of the Study:

  • To investigate the direct relationship between cell surface tension and cell surface motility.
  • To quantify the impact of externally applied and internally generated tension on cell motility domains.
  • To validate the role of tension as a global parameter controlling cell shape and motility.

Main Methods:

  • Utilized scanning acoustic microscopy (SAM) to image living cells over time.

Related Experiment Videos

  • Employed subtraction of sequential SAM images (SubSAM) to identify and analyze motility domains.
  • Manipulated cell tension by external stretching, internal contraction (nocodazole), and actin cytoskeleton relaxation (cytochalasin D).

    • Main Results:

    • SubSAM revealed metabolically dependent subcellular motility domains corresponding to cell surface deformations.
    • Elevated tension, irrespective of its source (external/internal, directed/isotropic), significantly reduced cell surface motility.
    • Relaxation of the cell cortex with cytochalasin D led to increased cell surface motility.

    Conclusions:

    • A direct inverse relationship between cell surface tension and cell surface motility was experimentally established.
    • Tension acts as a critical global regulator of cell surface dynamics and deformations.
    • Findings support previous hypotheses suggesting tension's role in controlling cell shape and motility.