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Enzymatic activity of aphroproteins.
N N Maksyutova1, I A Tarchevsky, D V Yusupova
1Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, 420503, Russia.
Biochemistry. Biokhimiia
|July 29, 1999
Summary
Larval foam excreted by Aphrophora costalis cicadas contains chitinase and proteinase enzymes, providing a fungicidal effect. This aphoprotein-based foam protects both the insect and host plant from fungal infections.
Area of Science:
- Entomology
- Biochemistry
- Plant Pathology
Background:
- Cicada larvae produce foam for protection.
- Fungal infections pose a threat to insects and plants.
Purpose of the Study:
- To investigate the fungicidal properties of aphroproteins excreted by Aphrophora costalis larvae.
- To identify the enzymatic activities responsible for the observed fungicidal effects.
Main Methods:
- Analysis of aphroproteins for enzymatic activity (chitinase, proteinase, DNase, RNase).
- Assessment of proteinase inhibitor properties.
Main Results:
- Aphroproteins exhibited significant chitinase and proteinase activities.
- No DNase or RNase activities were detected in the aphroproteins.
- Aphroproteins did not function as proteinase inhibitors.
Conclusions:
- The fungicidal effect of cicada larval foam is attributed to chitinase and proteinase activities in aphroproteins.
- Larval foam serves as a protective barrier against entomogenous and phytopathogenic fungi for the larva and host plant.