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Updated: Jul 18, 2026

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
Published on: May 7, 2018
Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells
Y Hayashida1, K Nakata, H Ishikawa
1First Department of Internal Medicine, Nagasaki University of Medicine, Nagasaki 852-8501, Japan.
Abstract:
Epidermal growth factor (EGF) and heparin-binding EGF-like growth factor (HB-EGF) exert their activities through the same cell surface receptor. In the present study, the role of EGF in cell kinetics of rat gastric epithelial cell line, RGM-1, in which HB-EGF is known to function in an autocrine fashion, was investigated. EGF stimulated the mucus glycoprotein synthesis which is characterized with cell maturation, but competed with HB-EGF for cell proliferation. By flow cytometric analysis, EGF inhibited G2/M transition by 48 h after stimulation and induced apoptosis manifested by hypodiploid DNA by 72 h after stimulation. Consistent with this, DNA ladder formation together with the histone-associated DNA fragments was found in EGF-treated cells. These results indicate that EGF and HB-EGF possess different functions in RGM-1 cells and that EGF acts as a mediator of both cell maturation and apoptosis in these cells.
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