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Related Experiment Videos

Biodegradable monodispersed nanoparticles prepared by pressure homogenization-emulsification.

A Lamprecht1, N Ubrich, M Hombreiro Pérez

  • 1Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté de Pharmacie, BP 403, 5 rue A. Lebrun, 54001, Nancy Cedex, France.

International Journal of Pharmaceutics
|July 30, 1999
PubMed
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Biodegradable nanoparticles (NP) were prepared using poly[D, L-lactic-co-glycolic acid] (PLGA) and poly[epsilon-caprolactone] (PCL) to encapsulate bovine serum albumin (BSA) protein drug carriers. PLGA nanoparticles demonstrated higher protein release (92%) compared to PCL nanoparticles (72%).

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Nanotechnology

Background:

  • Protein-based therapeutics require effective drug delivery systems.
  • Hydrophilic proteins like bovine serum albumin (BSA) present challenges for encapsulation due to high water solubility.
  • Biodegradable polymers offer potential for controlled protein release.

Purpose of the Study:

  • To investigate the preparation of biodegradable nanoparticles (NP) for protein drug delivery.
  • To encapsulate bovine serum albumin (BSA) as a model hydrophilic protein.
  • To compare the encapsulation efficiency and release profiles of PLGA and PCL nanoparticles.

Main Methods:

  • Utilized the double emulsion technique for nanoparticle preparation.
  • Employed a microfluidizer for homogenization to achieve submicron, monodisperse nanoparticles.

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  • Optimized drug loading by varying protein concentration, polymer concentration, surfactant concentration, and external phase volume.
  • Characterized encapsulation efficiency and in vitro release profiles.

    • Main Results:

    • Achieved high encapsulation efficiency for BSA (>80%) in both PLGA and PCL nanoparticles.
    • Observed a substantial initial burst release for both types of nanoparticles.
    • Poly[D, L-lactic-co-glycolic acid] (PLGA) nanoparticles showed a higher cumulative release (92%) compared to poly[epsilon-caprolactone] (PCL) nanoparticles (72%) at the end of the study.

    Conclusions:

    • Biodegradable nanoparticles, particularly PLGA, are effective carriers for hydrophilic proteins like BSA.
    • The double emulsion technique combined with microfluidization yields high-quality, monodisperse nanoparticles.
    • Release profiles are characterized by an initial burst release, with PLGA showing greater overall protein release than PCL.