Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neocortical projections regulate the neostriatal proenkephalin gene expression.

L Just1, C Olenik, D K Meyer

  • 1Department of Pharmacology, Albert-Ludwigs-University, Freiburg, Germany.

Cerebral Cortex (New York, N.Y. : 1991)
|July 30, 1999
PubMed
Summary

Neocortical projections regulate proenkephalin (PEnk) gene expression in the neostriatum. NMDA receptors and L-type Ca2(+) channels are crucial for this gene

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regional differences in pathogen spectra and antibiotic resistance in odontogenic abscesses: Comparison of two volume clinics.

Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2025
Same author

Spermatogonial stem cells from adult human testis and the generation of pluripotency in vitro and in vivo.

Journal of stem cells & regenerative medicine·2014
Same author

Frizzled-4 a potential neuronal marker of the enteric nervous system.

Journal of stem cells & regenerative medicine·2014
Same author

Embryonic stem cells in human sacrococcygeal teratomas: Isolation and characterization of an embryonic stem cell line.

Journal of stem cells & regenerative medicine·2014
Same author

Measurement of mRNA specific for preprocholecystokinin in rat caudatoputamen and areas projecting to it.

Neurochemistry international·2010
Same author

Release of cholecystokinin-like immunoreactivity from slices of dorsal and ventral striatum of rat brain.

Neurochemistry international·2010

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Gene Expression Regulation

Background:

  • Controversial evidence exists regarding the regulation of proenkephalin (PEnk) gene expression in the neostriatum by neocortical projections.
  • Understanding this regulation is crucial for deciphering neostriatal circuitry and function.

Purpose of the Study:

  • To investigate the role of neocortical projections in regulating PEnk gene expression within the neostriatum.
  • To elucidate the specific molecular mechanisms, including receptor involvement, mediating this regulation.

Main Methods:

  • Utilized organotypic neocortico-neostriatal co-cultures and isolated neostriatal cultures.
  • Quantified PEnk mRNA levels using in situ hybridization.
  • Investigated the effects of neocortex removal, NMDA receptor blockade, and nimodipine (L-type Ca2(+) channel blocker).

Related Experiment Videos

Main Results:

  • Neocortex removal led to a time-dependent decrease in PEnk gene expression in the neostriatum.
  • NMDA receptor blockade significantly reduced PEnk expression, indicating their mediating role.
  • NMDA stimulation in isolated slices, where PEnk expression was downregulated, increased PEnk expression in a time-dependent manner, which was attenuated by nimodipine.

Conclusions:

  • Neocortical projections play a significant role in regulating neostriatal PEnk gene expression.
  • NMDA receptors and L-type Ca2(+) channels are critical components of the signaling pathway involved in PEnk gene regulation.
  • The observed NMDA-induced PEnk expression increase suggests a specific neuronal recruitment process within the neostriatum.