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The spheroplast lysis assay for yeast in microtiter plate format
R Ovalle1, M Spencer, M Thiwanont
1Department of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021, USA.
Applied and Environmental Microbiology
|July 31, 1999
Abstract:
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.