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Updated: Oct 19, 2026
09:09
A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR
1Departamento de Microbiología, Universidad de Navarra, 31008 Pamplona, Spain.
Applied and Environmental Microbiology
|July 31, 1999
Abstract:
Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.
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