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An isolation procedure for arachidonic acid producing Mortierella species
1Department of Microbiology and Biochemistry, University of the Orange Free State, South Africa. abo@maties.sun.ac.za
Antonie Van Leeuwenhoek
|July 31, 1999
Abstract:
Malt extract agar and an incubation temperature of 5 degrees C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.