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Related Experiment Videos

Sensitivity testing of veterinary pathogens with a semi-automatic image analysis system compared with tablet

D J Mevius1, K T Veldman, R H van der Pal

  • 1Institute for Animal Science and Health (ID-DLO), Department of Bacteriology, AB Lelystad, The Netherlands. d.j.mevius@id.dlo.nl

The Veterinary Quarterly
|July 31, 1999
PubMed
Summary

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A new computer-controlled image analysis system (IAS) accurately measures antibiotic inhibition zones for most bacteria but shows significant differences in calculated minimum inhibitory concentrations (MICs). Clinical relevance of IAS MICs requires further investigation.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Pharmacology

Background:

  • Automated measurement systems are emerging for antimicrobial susceptibility testing.
  • The precision and clinical utility of computer-controlled image analysis systems (IAS) for agar diffusion tests require thorough evaluation.

Purpose of the Study:

  • To assess the accuracy and precision of a commercial IAS in measuring inhibition zone diameters.
  • To compare IAS-derived minimum inhibitory concentration (MIC) values with reference methods.
  • To evaluate the clinical relevance of discrepancies observed with the IAS.

Main Methods:

  • Clinical isolates of Salmonella spp., Escherichia coli, Pasteurella spp., Actinobacillus pleuropneumoniae, porcine streptococci, and Staphylococcus aureus were tested.
  • Inhibition zone diameters were measured manually by a technician and automatically by the IAS.

Related Experiment Videos

  • IAS-calculated MIC values were compared against reference agar dilution tests.

    • Main Results:

    • A significant positive correlation was found between IAS and technician measurements of zone diameters.
    • Discrepancies in zone diameter measurements occurred in 59% of combinations, though generally minor.
    • IAS-calculated MIC values correlated with reference values in 82% of cases but differed significantly in 92%, with potential clinical relevance.
    • IAS struggled with poor contrast for porcine streptococci inhibition zones.

    Conclusions:

    • The IAS accurately measures inhibition zones for bacteria with good agar contrast when using antibiotic tablets.
    • IAS-derived MIC values provide only an indication and may not be clinically reliable compared to reference methods.