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Characterisation of Streptococcus thermophilus CNRZ1205 and its cured and re-lysogenised derivatives
E Stanley1, G F Fitzgerald, D van Sinderen
1National Food Biotechnology Centre, University College, Cork, Ireland.
FEMS Microbiology Letters
|July 31, 1999
Abstract:
Streptococcus thermophilus CNRZ1205 is the lysogenic host for the temperate phage phi O1205. A derivative of CNRZ1205 was isolated which was cured of phi O1205 and this strain was used to construct a re-lysogenised derivative. Pulse field gel electrophoresis and sequencing of the attachment site regions confirmed that excision and re-integration of the phage was a site-specific event. Interestingly, cells from the cured, as well as its re-lysogenised derivative, were found to have a very long chain length.