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Updated: Jun 20, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos
Published on: April 11, 2011
Squid hnRNP protein promotes apical cytoplasmic transport and localization of Drosophila pair-rule transcripts
S Lall1, H Francis-Lang, A Flament
1Developmental Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Abstract:
Drosophila melanogaster pair-rule segmentation gene transcripts localize apically of nuclei in blastoderm embryos. This might occur by asymmetric (vectorial) export from one side of the nucleus or by transport within the cytoplasm. We have followed fluorescently labeled pair-rule transcripts postinjection into Drosophila embryos. Naked, microinjected fushi tarazu (ftz) transcripts do not localize in blastoderm embryos, indicating that cytoplasmic mechanisms alone are insufficient for apical targeting. However, prior exposure of ftz to Drosophila or human embryonic nuclear extract leads to rapid, specific, microtubule-dependent transport, arguing against vectorial export. We present evidence that ftz transcript localization involves the Squid (Hrp40) hnRNP protein and that the activity of hnRNP proteins in promoting transcript localization is evolutionarily conserved. We propose that cytoplasmic localization machineries recognize transcripts in the context of nuclear partner proteins.
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