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Updated: Aug 15, 2026
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
Culture media and their components differ in their ability to scavenge reactive oxygen species in the plasmid
A Ermilov1, M P Diamond, A G Sacco
1Department of Obstetrics and Gynecology, Hutzel Hospital/Wayne State University, Detroit, Michigan 48201, USA.
Objective:
To investigate the modulation of DNA-damaging effects of reactive oxygen species by media composition.
Design:
In vitro study.
Setting:
Academic medical center.
Patient(S):
None.
Intervention(S):
None.
Main Outcome Measure(S):
Plasmid relaxation.
Result(S):
Ham's F-10 medium, 1% Percoll, superoxide dismutase (1, 10, or 100 IU), and synthetic serum substitute did not affect DNA damage by reactive oxygen species and did not have any effect on plasmid DNA damage. Plasmid DNA damage was partially inhibited in the presence of P-1 and human tubal fluid media. Human serum albumin, phenol red, glucose, polyvinyl alcohol, polyvinylpyrrolidone, sucrose, and HEPES also were found to protect DNA from damage.
Conclusion(S):
In vitro fertilization media and their components vary widely in the way they affect DNA damage by reactive oxygen species.
