Existence of uncoupling protein-2 antigen in isolated mitochondria from various tissues

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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
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Published on: September 18, 2014

P Jezek1, M Zácková, Z Reháková

  • 1Department of Membrane Transport Biophysics, Institute of Physiology, Prague, Czech Republic. jezek@sun1.biomed.cas.cz

FEBS Letters
|July 31, 1999
PubMed

Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs. The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated.

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