Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatin assembly during DNA replication in somatic cells.

T Krude1

  • 1University of Cambridge, Wellcome/CRC Institute, UK. tk1@mole.bio.cam.ac.uk

European Journal of Biochemistry
|August 3, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noncoding human Y RNAs are overexpressed in tumours and required for cell proliferation.

British journal of cancer·2008
Same author

Reduction of chromatin assembly factor 1 p60 and C21orf2 protein, encoded on chromosome 21, in Down syndrome brain.

Journal of neural transmission. Supplementum·2004
Same author

Chromatin assembly during S phase: contributions from histone deposition, DNA replication and the cell division cycle.

Cellular and molecular life sciences : CMLS·2001
Same author

Viral cyclin-cyclin-dependent kinase 6 complexes initiate nuclear DNA replication.

Molecular and cellular biology·2001
Same author

Requirement of Cyclin/Cdk2 and protein phosphatase 1 activity for chromatin assembly factor 1-dependent chromatin assembly during DNA synthesis.

The Journal of biological chemistry·2000
Same author

Initiation of human DNA replication in vitro using nuclei from cells arrested at an initiation-competent state.

The Journal of biological chemistry·2000

Newly replicated DNA is assembled into chromatin via parental nucleosome segregation or de novo assembly mediated by chromatin assembly factors like CAF-1. This factor links new histones to DNA replication machinery during the S phase.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Epigenetics

Background:

  • DNA replication requires the assembly of newly synthesized DNA into chromatin.
  • Two primary pathways exist for chromatin assembly: parental nucleosome segregation and de novo assembly.

Purpose of the Study:

  • To elucidate the role of chromatin assembly factor 1 (CAF-1) in nucleosome assembly during DNA replication in somatic cells.

Main Methods:

  • Focuses on the function of chromatin assembly factors.
  • Investigates the interaction between histones and DNA replication machinery.

Main Results:

  • Chromatin assembly factor 1 (CAF-1) is crucial for de novo nucleosome assembly.
  • CAF-1 connects newly synthesized histones to the replication machinery.

Related Experiment Videos

  • This process occurs during the S phase of the cell cycle.

    • Conclusions:

    • CAF-1 plays a key role in ensuring proper chromatin formation post-DNA replication.
    • CAF-1 acts as a critical link between DNA synthesis and epigenetic inheritance.