Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A non-radioactive method for inexpensive quantitative RT-PCR.

M Maggiolini1, O Donzé, D Picard

  • 1Département de Biologie Cellulaire, Université de Genève, Switzerland.

Biological Chemistry
|August 3, 1999
PubMed
Summary

This study introduces a novel, non-radioactive quantitative reverse transcription polymerase chain reaction (RT-PCR) method. It offers high sensitivity and specificity for cDNA analysis, providing accurate results quickly and affordably.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic errors in peripheral facial palsy: A systematic review and meta-analysis.

European annals of otorhinolaryngology, head and neck diseases·2026
Same author

Impact of facial palsy impact on make-up habits: An observational cohort study.

European annals of otorhinolaryngology, head and neck diseases·2024
Same author

The development of a hiPSC-based platform to identify tissue-dependencies of IDH1 R132H.

Cell death discovery·2023
Same author

Publisher Correction: RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer.

Breast cancer research : BCR·2023
Same author

RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer.

Breast cancer research : BCR·2023
Same author

Contribution of the Synkinesis Assessment Questionnaire and the Sunnybrook Facial Grading System to the evaluation of synkinesis after peripheral facial palsy: A STROBE observational study.

European annals of otorhinolaryngology, head and neck diseases·2022

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Quantitative reverse transcription polymerase chain reaction (RT-PCR) is crucial for gene expression analysis.
  • Traditional methods may involve radioactivity or expensive equipment, limiting accessibility and throughput.
  • There is a need for sensitive, specific, and cost-effective quantitative RT-PCR techniques.

Purpose of the Study:

  • To develop and present a novel, non-radioactive method for quantitative RT-PCR.
  • To demonstrate the sensitivity, specificity, and quantitative accuracy of the new approach.
  • To offer a cost-effective and rapid alternative for molecular biology research.

Main Methods:

  • Direct incorporation of digoxigenin-11-dUTP (DIG-dUTP) during cDNA amplification in RT-PCR.

Related Experiment Videos

  • Separation of amplified products via agarose gel electrophoresis.
  • Southern transfer to a nylon membrane followed by chemiluminescent detection using an anti-DIG antibody.

    • Main Results:

    • The developed method is highly sensitive, enabling detection of low abundance targets.
    • Specificity is confirmed by monitoring the size of the RT-PCR product.
    • The procedure is quantitative, non-radioactive, and completed within approximately one day.

    Conclusions:

    • This novel DIG-dUTP based quantitative RT-PCR method provides a sensitive, specific, and rapid alternative.
    • The technique avoids radioactivity and expensive specialized equipment, making it broadly accessible.
    • It offers a reliable and cost-effective solution for quantitative gene expression analysis.