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[Experimental research on in vivo gene-therapy of retinoblastoma]
1Department of Ophthalmology, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu, 610041 P.R.China. dchen@oci.utoronto.ca
Objective:
To investigate the expression condition of exogenous Rb gene in nude mouse vitreous transplanted retinoblastoma(RB) and its influence on the growth of the RB tumor in vivo.
Methods:
Establishing the model of RB transplantation in nude mouse vitreous, followed by constructing the retrovirus vector of Rb gene and transfecting it into the model by using liposome. The expression condition of Rb gene was analyzed by immunohistochemistry and flow cytometry. The growth condition of the RB transplantation tumor was examined ophthalmologically and pathologically.
Results:
The exogenous Rb gene expressed in the RB transplantation tumor for at least 7 days and partially suppressed the growth of the RB tumor. The level of suppression was related with the amount of the Rb protein expressed and the time to transfect the Rb gene after the RB transplantation tumor was established.
Conclusion:
The exogenous Rb gene can express in the RB transplantation tumor and can partially suppress the growth of the RB tumor in vivo.