Related Experiment Videos
[A study on polymorphism of mitochondrial DNA D loop in the Han nationality in China]
1Department of Biology, Harbin Medical University, Harbin, 150086 P.R. China. zhusj@ems.hrbmu.edu.cn
Objective:
To investigate the polymorphism of mitochondrial DNA D loop in the Han nationality in China and the mean nucleic acid substitute frequency on each site.
Methods:
A 465bp-long DNA fragment in mtDNA D loop was amplified by PCR technique and the polymorphism was analyzed by restriction fragment length polymorphism (RFLP) using 6 restriction enzymes (on 10 sites). Some samples were analyzed by direct sequencing of PCR products.
Results:
RFLP analyses with 6 restriction enzymes on the 10 sites generated 14 restriction types. The calculated mean nucleic acid substitute frequency (based on Nei formula) on each site of mtDNA D loop in the Han nationality in China was 0.029.
Conclusion:
The mean nucleic acid substitute frequency on each site in the D loop region was 10 times as high as that in mtDNA.