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Updated: Oct 19, 2026
Electroporation of Plasmid DNA into Mouse Skeletal Muscle
Published on: April 6, 2022
[Gene transfer efficiency enhancement by inactivated Coxsakie virus B1 in primary skeletal muscle cells]
1Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University of Medical Science, Guangzhou, 510080 P.R.China. gjchen@gzsums.edu.cn
Objective:
To study the effect of inactivated Coxsackie virus B1(CVB1), which was considered to have high affinity to skeletal muscle cell, on gene transfer efficiency of reporter gene, pCDNA3-LacZ, in primary muscle cells.
Methods:
Viruses were purified by polyethylene sedimentation followed by sucrose supercentrifugation. Virus, transferrin-polylysine(TfpL) and LacZ were designed as different concentrations and groups, then positive cells contained beta-galactosidase were calculated as gene transfer efficiency.
Results:
Inactivated CVB1 made an enhancement of gene transfer efficiency from less than 1% in TfpL+LacZ group to 15-20 per cent in CVB1+TfpL+LacZ group.
Conclusion:
Like adenovirus, inactivated CVB1 can also increase gene transfer efficiency in muscle cells, which provides experimental basis for constructing new vector targeting to muscle cells.
