Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice

It is widely recognized that fluorescence techniques represent the most important future detection method for miniaturized ultra-high-throughput screening. However, such techniques encompass many different approaches, each with its own particular set of advantages and limitations. Here, the authors summarize each of the major fluorescence techniques and explain the underlying principles that form the basis for a theory-led strategy to readout selection and assay design.