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A YAC-based physical map of the mouse genome
C Nusbaum1, D K Slonim, K L Harris
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Nature Genetics
|August 4, 1999
Summary
Researchers created a detailed mouse genome physical map using sequenced-tagged sites (STSs) and yeast artificial chromosomes (YACs). This map provides extensive genomic coverage and facilitates positional cloning for biomedical research.
Area of Science:
- Genomics
- Mouse Genetics
- Bioinformatics
Background:
- A physical map of the mouse genome is crucial for positional cloning and sequencing in biomedical research.
- The Human Genome Project aims to create a mouse physical map with markers every 300 kb.
Purpose of the Study:
- To construct a high-resolution physical map of the mouse genome.
- To provide comprehensive genomic coverage for positional cloning and other studies.
Main Methods:
- Screening sequenced-tagged sites (STSs) against a yeast artificial chromosome (YAC) library.
- Integrating STS-content data with a dense genetic map.
- Developing a YAC-based map for chromosome X.
Main Results:
- An integrated map with 9,787 loci, average spacing of 300 kb, and 92% YAC coverage of the mouse genome.
- A YAC-based map of chromosome X with 619 loci, offering dense coverage.
- Facilitation of positional cloning of mouse mutations.
Conclusions:
- The developed physical map serves as a comprehensive framework for mouse genomic studies.
- This resource significantly aids in identifying genes responsible for mouse mutations.
- Combined with radiation hybrid maps, it enhances the study of the mouse genome.
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