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Anthocyanin extracts with antioxidant and radical scavenging effect
J Gabrielska1, J Oszmiański, M Komorowska
1Department of Physics and Biophysics, Agricultural University, Wrocław, Poland. jaga@ozi.ar.wroc.pl
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|August 4, 1999
Summary
Chokeberry, sloe, and honeysuckle anthocyanins show antioxidant activity, protecting liposome membranes from UV-induced oxidation. Chokeberry demonstrated the highest antioxidant efficiency, with potency dependent on extract concentration.
Area of Science:
- Biochemistry
- Food Science
- Pharmacology
Background:
- Anthocyanins are natural pigments with potential health benefits.
- Lipid oxidation, particularly in cell membranes, is linked to various diseases.
- UV radiation can induce oxidative stress in biological systems.
Purpose of the Study:
- To evaluate the antioxidative activity of anthocyanin pigments from chokeberry, honeysuckle, and sloe.
- To compare the efficacy of these anthocyanins in preventing UV-induced lipid oxidation in liposomes.
- To determine the concentration-dependent relationship of anthocyanin antioxidant potency.
Main Methods:
- Extraction of anthocyanin pigments from chokeberry, honeysuckle, and sloe fruits.
- Liposome membrane oxidation assay using thiobarbituric acid-reactive substances (TBARS) to measure lipid peroxidation.
- High-Performance Liquid Chromatography (HPLC) to identify oxidation end products.
- Electron Paramagnetic Resonance (EPR) technique to assess free radical scavenging capacity.
Main Results:
- The antioxidant efficiency followed the order: chokeberry > sloe > honeysuckle.
- Concentrations for 50% reduction in phosphatidylcholine oxidation were 48 mg/l (chokeberry), 54 mg/l (sloe), and 60 mg/l (honeysuckle).
- Antioxidative potency was found to be concentration-dependent for all extracts.
- Free radical elimination efficiency correlated with antioxidant activity, confirmed by EPR.
Conclusions:
- Anthocyanin extracts from chokeberry, sloe, and honeysuckle possess significant antioxidative properties.
- Chokeberry anthocyanins exhibit superior protection against UV-induced lipid oxidation compared to sloe and honeysuckle.
- The study confirms the concentration-dependent nature of anthocyanin antioxidant activity and their free radical scavenging capabilities.