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Related Experiment Videos

Invariant chain expression in colon neoplasms.

Z Jiang1, M Xu, L Savas

  • 1Department of Pathology, University of Massachusetts Medical Center, Worcester 01655, USA.

Virchows Archiv : an International Journal of Pathology
|August 4, 1999
PubMed
Summary

Invariant chain (Ii) expression increases with colon cancer grade, reducing tumor immunogenicity. High Ii levels correlate with fewer tumor-infiltrating lymphocytes (TILs), suggesting a suppressed immune response in advanced cancers.

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Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • Invariant chain (Ii) is a chaperone that inhibits antigen binding to MHC class II molecules.
  • Elevated Ii in cancer may reduce tumor antigen presentation, potentially decreasing immunogenicity.
  • Understanding Ii expression in colon cancer is crucial for assessing host immune response.

Purpose of the Study:

  • To correlate invariant chain (Ii) and HLA-DR expression in colon neoplasms with tumor grade.
  • To investigate the relationship between Ii, HLA-DR, and tumor-infiltrating lymphocyte (TIL) density in colon carcinomas.
  • To determine if Ii expression increases with colon cancer progression and affects tumor immunogenicity.

Main Methods:

  • Immunohistochemical staining of surgical specimens from 48 patients for Ii and HLA-DR.

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  • Grading of tumor differentiation (WDAC, MDAC, PDAC) and adenomas (AdHGD).
  • Quantification of TIL aggregates in H&E-stained sections.

    • Main Results:

    • Normal colon epithelium was negative for Ii and HLA-DR.
    • Ii expression increased linearly with tumor grade progression (low to high-grade).
    • HLA-DR expression showed no significant difference across tumor grades.
    • A disproportionate increase in Ii over HLA-DR was observed in invasive carcinomas.
    • TIL frequency was inversely correlated with Ii expression and tumor grade.

    Conclusions:

    • This study is the first to demonstrate increased Ii expression during colon neoplasm progression, most notably in poorly differentiated carcinomas.
    • Increased Ii expression in colon carcinomas is inversely related to TIL frequency.
    • Elevated Ii expression likely renders tumors less immunogenic, diminishing the host immune response.