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Screening for genes up-regulated in 5/6 nephrectomized mouse kidney
1Department of Medicine III, Okayama University Medical School, Japan.
Kidney International
|August 5, 1999
Summary
Researchers identified 19 genes, including novel ones, involved in kidney hyperfiltration and hypertrophy after partial nephrectomy in mice. These findings shed light on the early stages of progressive renal injury.
Area of Science:
- Nephrology
- Molecular Biology
- Genetics
Background:
- Glomerular hyperfiltration is a key factor in diabetic and nondiabetic renal diseases, driving glomerulosclerosis and scarring.
- Identifying genes involved in hyperfiltration and hypertrophy is crucial for understanding renal disease progression.
Purpose of the Study:
- To identify genes associated with glomerular hyperfiltration and hypertrophy in a mouse model of renal disease.
- To utilize representational difference analysis of cDNA (cDNA-RDA) for gene discovery.
Main Methods:
- Surgical 5/6 nephrectomy was performed on 10-week-old ICR mice, with sham-operated mice serving as controls.
- Messenger RNAs (mRNAs) were isolated from control and remnant kidneys two weeks post-surgery.
- Complementary DNA (cDNA) representational difference analysis (RDA) was employed to identify differentially expressed genes.
Main Results:
- Representational difference analysis of cDNA identified 19 genes (10 known, 9 novel) potentially involved in renal injury.
- Northern blot analysis confirmed 1.5- to 6-fold up-regulation in 12 clones (8 known, 4 novel).
- Known genes identified suggest roles for androgen action, mitochondrial function, matrix metabolism, and cell signaling in early renal injury.
Conclusions:
- The identified known and novel genes are implicated in the initial hyperfiltration and hypertrophy observed in remnant kidneys.
- This study highlights the utility of cDNA-RDA in discovering genes relevant to the pathobiology of progressive renal injury.