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Uniform band intensities in fluorescent dye terminator sequencing
S Kumar1, C W Fuller, S Nampalli
1Amersham Pharmacia Biotech, Cleveland, OH 44128, USA.
Nucleosides & Nucleotides
|August 5, 1999
Abstract:
The use of Cyanine dye (Cy5 and Cy5.5) labeled dideoxy terminators with Thermo Sequenase DNA polymerase in DNA sequencing provides uniform band intensity, improved sequence read-length, and accuracy. It also greatly improves the ability to detect single base heterozygotes with dye-terminator sequencing method.