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Conductances, diffusion and streaming potentials in the rat proximal tubule.

G B De Mello, A G Lopes, G Malnic

    The Journal of Physiology
    |September 1, 1976
    PubMed
    Summary
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    Investigating rat proximal tubules, this study found that intercellular spaces are the primary ion pathway. Hypotonic perfusion revealed negatively charged pores, influencing ion transport and tubule resistance.

    Area of Science:

    • Nephrology
    • Renal Physiology
    • Cell Biology

    Background:

    • The proximal tubule is crucial for reabsorption in the kidney.
    • Understanding its ion transport mechanisms is vital for renal function.
    • Previous studies have suggested intercellular spaces play a role in transport.

    Purpose of the Study:

    • To measure transtubular potential differences and specific resistances in rat proximal tubules.
    • To elucidate the primary pathways for ion transport across the proximal tubule.
    • To characterize the nature of pores involved in ion movement.

    Main Methods:

    • Utilized single and double-barreled glass microelectrodes for precise measurements.
    • Employed oil droplet passage and choline chloride perfusion for accurate electrode tip localization.

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  • Performed luminal and peritubular perfusions with varying solutions to assess ion conductances and streaming potentials.

    • Main Results:

    • Recorded mean early proximal potential differences (p.d.s.) of -1 to -2 mV and late values of +0.5 to +1 mV.
    • Determined mean specific resistances ranging from 12 to 15 omega cm².
    • Identified similar sodium (Na) and potassium (K) conductances, both greater than chloride (C1) conductance.
    • Observed streaming potentials indicating negatively charged pores, with hypotonic perfusion increasing resistance.

    Conclusions:

    • Intercellular spaces represent the main ion transport pathway in rat proximal tubules.
    • The presence of negatively charged pores influences ion movement and tubule resistance.
    • Findings provide insights into the structural and electrical properties governing proximal tubule function.