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Interplay between nitric oxide and CGRP by capsaicin in isolated guinea-pig heart
G Oroszi1, Z Szilvassy, J Nemeth
1Department of Pharmacology and Pharmacotherapy, Medical University of Pecs and Neuropharmacology Research Group of the Hungarian Academy of Sciences, Pecs, H-7643, Hungary.
Abstract:
Capsaicin at a concentration of 10(-7)m induced a significant increase in heart rate and increased coronary flow in isolated Langendorff-perfused guinea-pig hearts. This effect was completely blocked by 30 microm of N(omega)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase. Additional incubation with 3 m m L-Arg antagonized the inhibitory effect of L-NAME. In the presence of 1 microm of a human calcitonin gene-related peptide fragment (hCGRP 8-37), a CGRP-receptor antagonist, L-Arg was without effect. We conclude that a capsaicin-induced increase in coronary flow and heart rate is dependent from an interplay between CGRP and NO in guinea-pig hearts. 1999 Academic Press.