Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ocular cell monolayers cultured on biodegradable substrates.

T Hadlock1, S Singh, J P Vacanti

  • 1Department of Surgery, Children's Hospital, and Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Boston, MA, USA.

Tissue Engineering
|August 6, 1999
PubMed
Summary

Researchers successfully cultured retinal pigment epithelial (RPE) and corneal endothelial cells on biodegradable polymers. These findings support potential applications in cell monolayer transplantation for vision restoration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Organ Replacement, The New Technology of Tissue Engineering.

Surgical technology international·2017
Same author

Differentiation of human bone marrow mesenchymal stem cells on decellularized extracellular matrix materials.

Journal of biomedical materials research. Part A·2013
Same author

Tissue engineering and the road to whole organs.

The British journal of surgery·2012
Same author

Methods for the implantation of liver cells.

Methods in molecular medicine·2011
Same author

Hepatocyte transplantation in the dalmatian dog model of hyperuricosuria.

Tissue engineering·2009
Same author

Hepatocyte transplantation in Swine using prevascularized polyvinyl alcohol sponges.

Tissue engineering·2009

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Cell Biology

Background:

  • Retinal pigment epithelial (RPE) and corneal endothelial cells are crucial for vision.
  • Current transplantation methods face challenges.
  • Biodegradable substrates offer potential for improved cell delivery and integration.

Purpose of the Study:

  • To culture RPE and corneal endothelial cells on biodegradable polymers (PLLA and PLGA).
  • To evaluate cell density, viability, and structural integrity on these substrates.
  • To assess the potential for these cultured cell monolayers in future ocular transplantation.

Main Methods:

  • Culturing human/pig RPE cells and rabbit corneal endothelial cells on poly-l-lactic acid (PLLA) and poly-dl-lactic-co-glycolic acid (PLGA) films.

Related Experiment Videos

  • Assessing cell density and viability post-culture.
  • Immunohistochemical staining for F-actin and zonula occludens protein 1 (ZO-1) to confirm cell structure and tight junction formation.

    • Main Results:

    • Achieved successful culture of RPE and corneal endothelial cell monolayers on PLLA and PLGA substrates.
    • Demonstrated high cell densities: human RPE on PLLA (2950 cells/mm²), pig RPE on PLLA/PLGA (2350 cells/mm²), and rabbit corneal cells (2650 cells/mm²).
    • Confirmed the presence of intact actin filaments and tight junctions (ZO-1) in all cultured cell types.

    Conclusions:

    • Biodegradable PLLA and PLGA polymers support the successful culture of functional RPE and corneal endothelial cell monolayers.
    • These findings indicate a promising potential for using these cultured cell monolayers in ocular transplantation strategies.
    • This approach may offer a viable method for vision restoration through cell-based therapies.