Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An animal model to study microcirculatory changes associated with vascular delay.

J H Barker1, J Frank, S B Bidiwala

  • 1Division of Plastic and Reconstructive Surgery, University of Louisville, KY, USA.

British Journal of Plastic Surgery
|August 6, 1999
PubMed
Summary

Vascular delay, a surgical technique, significantly reduces flap necrosis in hairless mouse ears. This study demonstrates a minimum 6-day delay is needed for significant benefits, highlighting microcirculation changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Making Informed Choices On Incorporating Chemoprevention into carE (MiCHOICE, SWOG 1904): Design and methods of a cluster randomized controlled trial.

Contemporary clinical trials·2024
Same author

Continuous Combined Estrogen Plus Progestin and Endometrial Cancer: The Women's Health Initiative Randomized Trial.

Journal of the National Cancer Institute·2015
Same author

Reliable evidence from placebo-controlled, randomized, clinical trials for menopausal hormone therapy's influence on incidence and deaths from breast cancer.

Climacteric : the journal of the International Menopause Society·2015
Same author

Of conflicts, conspiracies, red herrings, and black swans.

Climacteric : the journal of the International Menopause Society·2015
Same author

Health risks and benefits from calcium and vitamin D supplementation: Women's Health Initiative clinical trial and cohort study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2012
Same author

Critical-oxygen tension of Caenorhabdiltis elegans.

Journal of nematology·2009

Area of Science:

  • Surgical Innovation
  • Microcirculation Research
  • Animal Models in Medicine

Background:

  • Vascular delay is a surgical procedure to improve flap viability by inducing partial ischemia before transfer.
  • The precise mechanisms of vascular delay are debated, but microcirculatory changes are considered crucial.
  • Understanding these microcirculatory adaptations is key to optimizing reconstructive surgery outcomes.

Purpose of the Study:

  • To establish the hairless mouse ear as a model for studying vascular delay-induced microcirculatory changes.
  • To quantify the reduction in flap necrosis following vascular delay.
  • To determine the optimal delay period and the role of specific neurovascular components.

Main Methods:

  • Utilized homozygous (hr/hr) hairless mice for direct observation of microcirculation.

Related Experiment Videos

  • Performed vascular delay procedures on mouse ears, varying delay duration and neurovascular pedicle ligation.
  • Assessed flap necrosis and measured vessel directionality changes post-delay.

    • Main Results:

    • Vascular delay significantly reduced ear flap necrosis compared to controls (P < 0.05).
    • A minimum delay of 6 days was required for statistically significant reduction in necrosis.
    • Ligation of artery, vein, or nerve individually, or in combination, all significantly reduced necrosis.
    • Significant changes in vessel directionality were observed starting at 6 days of delay.

    Conclusions:

    • The hairless mouse ear is an effective model for studying vascular delay.
    • A delay period of at least 6 days is critical for achieving significant reduction in flap necrosis.
    • Microcirculatory changes, including altered vessel directionality, play a key role in the efficacy of vascular delay.