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Production of CTC-containing porcine reference materials
J D McEvoy1, J P Ferguson, S R Crooks
1Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Stormont, Belfast, UK. John.McEvoy@dani.gov.uk
The Analyst
|August 6, 1999
Summary
Reference materials for chlortetracycline (CTC) in pig tissues were developed to harmonize veterinary drug residue testing in the EU. These materials were found to be homogenous, stable, and suitable for certification, aiding accurate analysis.
Area of Science:
- Food Safety
- Analytical Chemistry
- Veterinary Pharmacology
Background:
- The European Commission's Standards, Measurements and Testing programme aims to harmonize veterinary drug residue testing across the EU.
- Standardized Reference Materials (RMs) are crucial for accurate and comparable analytical results in food safety testing.
Purpose of the Study:
- To produce chlortetracycline (CTC)-free and CTC-incurred pig tissues as candidate Reference Materials (RMs).
- To ensure the homogeneity, stability, and suitability of these candidate RMs for certification.
Main Methods:
- Production of CTC-free and CTC-incurred pig kidney, liver, and muscle tissues.
- Analysis using High-Performance Liquid Chromatography (HPLC) with fluorescence detection for CTC and 4-epi-CTC.
- Assessment of homogeneity and stability under various storage conditions and timeframes.
Main Results:
- CTC-incurred lyophilised pig tissues (kidney, liver, muscle) were produced with mean CTC concentrations of 1,315, 765, and 378 µg kg⁻¹, respectively.
- High homogeneity was confirmed with relative standard deviations (RSDs) below 5% for all tissue types.
- No measurable degradation was observed over 79 weeks of storage at temperatures ranging from -18 to 37°C, indicating excellent stability.
Conclusions:
- The produced CTC-free and CTC-incurred pig tissue candidate RMs are homogenous and stable.
- These materials are suitable for certification and will aid in harmonizing veterinary drug residue testing within the EU.