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Thyroid hormone regulates reelin and dab1 expression during brain development.

M Alvarez-Dolado1, M Ruiz, J A Del Río

  • 1Instituto de Investigaciones Biomédicas "Alberto Sols", Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, 28029 Madrid, Spain.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|August 6, 1999
PubMed
Summary

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Thyroid hormone influences brain development by regulating reelin and dab1 gene expression, crucial for neuronal migration. This study reveals how thyroid hormone deprivation and administration impact these genes during critical developmental periods.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Endocrinology

Background:

  • Reelin and dab1 genes are essential for neuronal migration and brain development.
  • Thyroid hormone plays a critical role in regulating these developmental processes.

Purpose of the Study:

  • To investigate the impact of thyroid hormone on the expression of reelin and dab1 genes.
  • To understand the role of these genes in thyroid hormone-mediated neuronal development.

Main Methods:

  • Northern analysis, in situ hybridization, and immunohistochemistry were used to assess gene and protein expression.
  • Experiments were conducted on hypothyroid rats and cultured hippocampal slices.
  • In vivo and in vitro hormone administration was performed to confirm thyroid-dependent regulation.

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Main Results:

  • Hypothyroidism led to decreased reelin RNA and protein levels during the perinatal period (E18, P0) in specific brain regions.
  • Reelin levels were higher in hypothyroid rats at P5, while Dab1 levels showed inverse changes.
  • Thyroid hormone treatment increased reelin expression both in vivo and in vitro.

Conclusions:

  • Thyroid hormone regulates the expression of reelin and dab1 during brain development.
  • These genes may mediate some of the effects of thyroid hormone on neuronal migration.
  • Findings highlight a molecular link between thyroid hormone and key developmental genes in the brain.