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The sae locus of Staphylococcus aureus encodes a two-component regulatory system
A T Giraudo1, A Calzolari, A A Cataldi
1Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico, Química y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Abstract:
Sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment was shown to carry the sae locus by restoration of exoprotein production in sae mutants. The sae locus was mapped to the SmaI-D fragment of the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the cloned fragment revealed the presence of two genes, designated saeR and saeS, encoding a response regulator and a histidine protein kinase, respectively, with high homology to other bacterial two-component regulatory systems.