Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vertebrate exon trapping methods: implications for transcript mapping with mosquito DNA.

S E Brown1, S H Wood, D L Knudson

  • 1Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins 80523, USA.

Insect Biochemistry and Molecular Biology
|August 7, 1999
PubMed
Summary

Vertebrate exon trapping methods can identify mosquito exons, but struggle with short introns (< 63 bp). This impacts its use in mosquito genomics and positional cloning efforts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting Urban Heat-Related Illness Across U.S. Climate Regions and Demographics.

GeoHealth·2026
Same author

A comparative study of pharyngolaryngectomy free flap reconstruction: A 12-year retrospective analysis at a single center.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2025
Same author

Improved nitrogen fertilizer management reduces nitrous oxide emissions in a northern Prairie cropland.

The Science of the total environment·2024
Same author

First Application of Whole Genome Sequencing in Myelinated Retinal Nerve Fibers (MRNF).

Physiological research·2024
Same author

Risk characterization of N-nitrosodimethylamine in pharmaceuticals.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2024
Same author

Controlled Frustration Release on the Kagome Lattice by Uniaxial-Strain Tuning.

Physical review letters·2024

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Exon trapping is crucial for transcript map development.
  • In vivo vertebrate exon trapping recovers exons from genomic DNA clones.
  • Successful trapping relies on vertebrate splice site recognition.

Purpose of the Study:

  • To assess the efficacy of vertebrate exon trapping for mosquito genomics.
  • To determine if invertebrate splice sites are processed by vertebrate exon trapping methods.
  • To evaluate the application of exon trapping in mosquito map-based positional cloning.

Main Methods:

  • Bioinformatic analysis of mosquito splice sites using exon identification software.
  • Experimental testing of a mosquito genomic DNA clone (D7 gene) with in vivo vertebrate exon trapping.

Related Experiment Videos

  • Analysis of recovered exons and exon fragments.

    • Main Results:

    • Bioinformatic analysis suggested potential recognition of mosquito introns and exons by the vertebrate method.
    • Experimental trapping failed to faithfully process mosquito introns smaller than 63 bp.
    • Exons and exon fragments were successfully recovered despite the intron processing limitations.

    Conclusions:

    • The vertebrate exon trapping method shows partial applicability to mosquito genomics.
    • Limitations exist in processing short invertebrate introns, affecting accuracy.
    • Findings inform the application of exon trapping for mosquito map-based positional cloning.