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Updated: Oct 19, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
Synthesis and immunologic characterisation of Mycobacterium tuberculosis lipoarabinomannan specific
B Hamasur1, G Källenius, S B Svenson
1Swedish Institute for Infectious Disease Control, Stockholm.
Abstract:
Lipoarabinomannan (LAM) is a major structural surface component of all mycobacteria, and has been reported to have a wide range of biological effects. Immunogenic LAM specific oligosaccharide protein conjugates were synthesized and immunologically characterized. Oligosaccharides were derived from LAM purified from Mycobacterium tuberculosis H37 Rv and covalently conjugated to tetanus toxoid and cross reactive mutant (CRM197) diphtheria toxoid. Both types of LAM oligosaccharide protein conjugates proved to be highly immunogenic, inducing a boosterable T helper cell dependent IgG response. These conjugates are currently evaluated as components in a subcellular experimental TB vaccine.
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