Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The presence of human immunodeficiency virus type 1 Vpr correlates with a decrease in the frequency of mutations in a

J B Jowett1, Y M Xie, I S Chen

  • 1Departments of Microbiology & Immunology and Medicine, University of California-Los Angeles School of Medicine, Los Angeles, California 90095-1678, USA.

Journal of Virology
|August 10, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of 20 cases of primary coarctation of the aorta in children treated with transcatheter covered stent implantation].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[Intermediate and long-term outcomes of transcatheter closure of congenital coronary cameral fistulas in 66 children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2024
Same author

Laparoscopic preperitoneal repair for primary falciform ligament herniation.

Annals of the Royal College of Surgeons of England·2020
Same author

The appropriate hybrid surgical strategy in three-level cervical degenerative disc disease: a finite element analysis.

Journal of orthopaedic surgery and research·2019
Same author

Gastrointestinal: Ileal Dieulafoy's lesion presenting as a bleeding submucosal tumor.

Journal of gastroenterology and hepatology·2016
Same author

Inguinal Hernia: Recurrences, Tailored Surgery & Pubic Inguinal Pain Syndrome (Sportsman Hernia).

Hernia : the journal of hernias and abdominal wall surgery·2015

The human immunodeficiency virus type 1 (HIV-1) Vpr protein influences DNA repair mechanisms. HIV-1 Vpr presence reduced DNA deletions, suggesting a role in viral replication and pathogenesis.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • The human immunodeficiency virus type 1 (HIV-1) Vpr protein is known to induce cell cycle arrest at the G2/M phase.
  • This arrest is similar to that caused by DNA-damaging agents, raising questions about Vpr's effect on DNA repair.

Purpose of the Study:

  • To investigate the impact of the HIV-1 Vpr protein on cellular DNA repair capabilities.
  • To determine if Vpr affects the repair of UV-damaged DNA.

Main Methods:

  • Development of a shuttle vector system to assess DNA repair efficiency.
  • Analysis of UV-damaged DNA repair rates in the presence of Vpr.
  • Comparison with DNA repair in cells arrested by other genotoxic agents.

Main Results:

Related Experiment Videos

  • The presence of HIV-1 Vpr decreased the rate of deletions in the shuttle vector system.
  • Cells arrested in G2 by other genotoxic agents showed an increased frequency of UV-damaged shuttle vector repair.
  • No direct effect of Vpr on double-strand break repair or nucleotide excision repair of genomic DNA was observed.

Conclusions:

  • HIV-1 Vpr appears to alter the frequency of DNA repair, specifically reducing deletions.
  • This modulation of DNA repair by Vpr may play a significant role in HIV-1 replication and the pathogenesis of the virus.