Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intracellular rate-limiting steps in MHC class I antigen processing.

M Montoya1, M Del Val

  • 1Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Madrid, Spain.

Journal of Immunology (Baltimore, Md. : 1950)
|August 10, 1999
PubMed
Summary

Increasing antigen (Ag) synthesis sharply boosts CD8+ cytotoxic T lymphocyte (CTL) recognition, but high Ag levels cause saturation. The rate-limiting step for Ag presentation involves peptide binding to MHC molecules within infected cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ELECLA trial: final results of perioperative chemotherapy with fluoropyrimidine and oxaliplatin in mismatch repair proficient locally advanced colon cancer.

ESMO open·2026
Same author

Low thermal inertia of carbonaceous asteroid Bennu driven by cracks observed in returned samples.

Nature communications·2026
Same author

Clinical Complete Response and Organ Preservation Strategies in Rectal Cancer: A Real-World Single-Center Experience Clinical Complete Response and Organ Preservation in Rectal Cancer.

Cancers·2026
Same author

Gel nail polish does not have a negative impact on the nail bacterial burden nor on the quality of hand hygiene with an alcohol-based hand rub.

The Journal of hospital infection·2024
Same author

Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella.

Nature communications·2022
Same author

Low-dose radiotherapy to the lungs using an interventional radiology C-arm fluoroscope: Monte Carlo treatment planning and dose measurements in a postmortem subject.

Biomedical physics & engineering express·2022

Area of Science:

  • Immunology
  • Molecular Biology
  • Virology

Background:

  • Understanding antigen processing and presentation by MHC class I molecules to CD8+ cytotoxic T lymphocytes (CTLs) is crucial for adaptive immunity.
  • The endogenous pathway dictates how intracellular antigens are processed and displayed on the cell surface for immune surveillance.

Purpose of the Study:

  • To quantitatively analyze the relationship between antigen (Ag) expression levels and CTL recognition via the endogenous pathway.
  • To identify the rate-limiting steps in antigen processing and presentation by MHC class I molecules in vaccinia virus-infected cells.

Main Methods:

  • Utilized recombinant vaccinia virus expressing beta-galactosidase as a model antigen in infected cells.
  • Varied the amount of starting antigen synthesis while keeping other factors constant.

Related Experiment Videos

  • Assessed antigen presentation and subsequent CD8+ CTL recognition across a range of antigen expression levels.

    • Main Results:

    • A sharp increase in CTL recognition was observed as antigen synthesis increased, up to a certain threshold.
    • Higher levels of antigen expression resulted in a saturation point, limiting the number of peptides bound to MHC molecules intracellularly.
    • This saturation ultimately constrained the level of CTL recognition.

    Conclusions:

    • The binding of antigenic peptides to MHC class I molecules, occurring within the infected cell or preceding this step, represents the rate-limiting factor in the endogenous antigen presentation pathway.
    • Antigen processing and presentation are subject to intracellular saturation, impacting T cell-mediated immune responses.