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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
Interleukin-8 stimulates the adhesion of endometrial stromal cells to fibronectin
1Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut 06520-8063, USA.
Objective:
To study the effect of interleukin-8 (IL-8) and mouse anti-human IL-8 monoclonal antibody on endometrial stromal cell adhesion to fibronectin.
Design:
Prospective, controlled in vitro study.
Setting:
Academic research laboratory.
Patient(S):
Women undergoing benign gynecologic surgery for indications other than endometrial disease.
Intervention(S):
Endometrial stromal cells were treated with IL-8 and mouse anti-human IL-8 monoclonal antibody at different concentrations and adhesion assays were performed by labeling the cells with calcein.
Main Outcome Measure(S):
Fluorescence in calcein-labeled endometrial stromal cells.
Result(S):
IL-8 induced a concentration-dependent increase of cell adhesion to fibronectin matrix (twofold at 10 ng/mL; P = .006). Treatment of endometrial stromal cells with anti-IL-8 neutralizing antibody inhibited partially the cell adhesion.
Conclusion(S):
IL-8 stimulates the adhesion of endometrial stromal cells to fibronectin in a concentration-dependent manner. IL-8 may thus be relevant for the attachment of endometrial implants in the pathogenesis of endometriosis.
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