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Cloning the laboratory mouse.

T Wakayama1, R Yanagimachi

  • 1Department of Anatomy and Reproductive Biology, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA.

Seminars in Cell & Developmental Biology
|August 12, 1999
PubMed
Summary
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Researchers cloned laboratory mice using cumulus cell nuclei, achieving normal development over four generations. However, the low success rate (1%) highlights challenges in nuclear transfer efficiency and genomic reprogramming for successful mammalian cloning.

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Genetics

Background:

  • Mammalian cloning has evolved from using preimplantation embryo cells to adult somatic cells.
  • Recent successes include cloning sheep, cattle, and mice using various adult cell types.

Purpose of the Study:

  • To develop and assess a technique for cloning laboratory mice using adult somatic cells.
  • To investigate the efficiency and developmental outcomes of nuclear transfer from cumulus cells.

Main Methods:

  • Nuclear transfer of cumulus cells into enucleated oocytes.
  • Generation of cloned mouse populations and assessment of their development and fertility over multiple generations.

Main Results:

  • Successfully produced over 80 cloned mice using cumulus cell nuclear transfer.

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  • Cloned animals exhibited normal development and fertility across four generations.
  • Achieved a low overall yield, with only approximately 1% of injected oocytes resulting in live offspring.

    • Conclusions:

    • Cumulus cell nuclear transfer is a viable method for producing cloned mice with normal development.
    • The extremely low success rate indicates significant challenges in the technique, possibly related to genomic reprogramming, somatic mutations, imprinting, or cell cycle synchronization.