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Generation of Mice Derived from Induced Pluripotent Stem Cells
Published on: November 29, 2012
Cloning the laboratory mouse
1Department of Anatomy and Reproductive Biology, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA.
Seminars in Cell & Developmental Biology
|August 12, 1999
Summary
Researchers cloned laboratory mice using cumulus cell nuclei, achieving normal development over four generations. However, the low success rate (1%) highlights challenges in nuclear transfer efficiency and genomic reprogramming for successful mammalian cloning.
Area of Science:
- Reproductive biology
- Developmental biology
- Genetics
Background:
- Mammalian cloning has evolved from using preimplantation embryo cells to adult somatic cells.
- Recent successes include cloning sheep, cattle, and mice using various adult cell types.
Purpose of the Study:
- To develop and assess a technique for cloning laboratory mice using adult somatic cells.
- To investigate the efficiency and developmental outcomes of nuclear transfer from cumulus cells.
Main Methods:
- Nuclear transfer of cumulus cells into enucleated oocytes.
- Generation of cloned mouse populations and assessment of their development and fertility over multiple generations.
Main Results:
- Successfully produced over 80 cloned mice using cumulus cell nuclear transfer.
- Cloned animals exhibited normal development and fertility across four generations.
- Achieved a low overall yield, with only approximately 1% of injected oocytes resulting in live offspring.
Conclusions:
- Cumulus cell nuclear transfer is a viable method for producing cloned mice with normal development.
- The extremely low success rate indicates significant challenges in the technique, possibly related to genomic reprogramming, somatic mutations, imprinting, or cell cycle synchronization.
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