Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1).

R Kakkar1, R V Raju, R K Sharma

  • 1Department of Pathology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.

Cellular and Molecular Life Sciences : CMLS
|August 12, 1999
PubMed
Summary

Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) isozymes are regulated by calpains, impacting cellular signaling. Understanding PDE1A2

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute renal failure associated with liver disease in India: etiology and outcome.

Renal failure·2000
Same author

Impairment of the rod outer segment membrane guanylate cyclase dimerization in a cone-rod dystrophy results in defective calcium signaling.

Biochemistry·2000
Same author

Coinfection with leprosy and tuberculosis in a renal transplant recipient.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2000
Same author

Improvement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing.

Human reproduction (Oxford, England)·2000
Same author

Survival of long-term retinal cell transplants.

Acta ophthalmologica Scandinavica·2000
Same author

Effect of treatment on PCR positivity in multibacillary leprosy patients treated with conventional and newer drugs ofloxacin and minocycline.

Acta leprologica·2000

Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Signaling
  • Enzymology

Background:

  • Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) enzymes are crucial in cyclic nucleotide and Ca2+ signaling.
  • Multiple PDE1 isozymes exist, generated by alternative splicing, with distinct regulatory properties.
  • PEST motifs, rich in proline, glutamate, serine, and threonine, signal for rapid protein degradation by calpains.

Purpose of the Study:

  • To describe the regulation of PDE1A2 by calpains and explore its physiological implications.
  • To highlight the importance of time-dependent regulatory reactions in cell activation.
  • To emphasize the need for in vivo studies to clarify PDE1 isozyme functions in pathophysiological processes.

Main Methods:

  • Analysis of Ca2+ and cyclic adenosine monophosphate (cAMP) regulatory reactions.

Related Experiment Videos

  • Identification of PDE1A2 as a substrate for m-calpain.
  • Review of current understanding of PDE1 inhibitors and their roles.

    • Main Results:

    • Isozyme PDE1A2 possesses a PEST motif and is regulated by m-calpain.
    • Differential regulation by Ca2+, calmodulin, and phosphorylation exists among PDE1 isozymes.
    • cAMP signaling pathways are central to crosstalk regulation by various signaling pathways.

    Conclusions:

    • Calpain-mediated regulation of PDE1A2 has significant physiological implications.
    • Understanding PDE1 isozyme function and cAMP response alterations is key to disease pathology and therapeutics.
    • Further in vivo research is essential to elucidate the exact functions of PDE1 isozymes in pathophysiological processes.