Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extra-epididymal spermatozoa express nuclear abnormalities.

R J Potts1, L J Notarianni, T M Jefferies

  • 1School of Pharmacy and Pharmacology, University of Bath, UK.

International Journal of Andrology
|August 12, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of dioxin and dioxin-like compounds in mainstream smoke from selected US cigarette brands and reference cigarettes.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2008
Same author

Mechanisms regulating the cadmium-mediated suppression of Sp1 transcription factor activity in alveolar epithelial cells.

Toxicology·2002
Same author

Inhibition of oxidative DNA repair in cadmium-adapted alveolar epithelial cells and the potential involvement of metallothionein.

Toxicology·2001
Same author

Cadmium-mediated oxidative stress in alveolar epithelial cells induces the expression of gamma-glutamylcysteine synthetase catalytic subunit and glutathione S-transferase alpha and pi isoforms: potential role of activator protein-1.

Cell biology and toxicology·2001
Same author

Cadmium adaptation in the lung - a double-edged sword?

Toxicology·2001
Same author

Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation.

Mutation research·2001
Same journal

News from the European Academy of Andrology (EAA).

International journal of andrology·2018
Same journal

The EAA International Quality Control Programme for Y-Chromosomal Microdeletions.

International journal of andrology·2018
Same journal

Cell surface molecules of human teratoma cell lines.

International journal of andrology·2017
Same journal

Foreword.

International journal of andrology·2017
Same journal

Discussion.

International journal of andrology·2017
Same journal

Discussion.

International journal of andrology·2017
See all related articles

Spermatozoa outside the testes experience DNA and chromatin damage. This study found higher abnormalities in vasectomized men, explaining reduced sperm viability and fertilizing capacity in extra-epididymal sperm.

Area of Science:

  • Reproductive Biology
  • Spermatozoa Biology
  • Genetics

Background:

  • Extra-epididymal spermatozoa, comprising about one-third of ejaculate, lose functional competence at core body temperature.
  • Spermatozoa persisting outside the testes and epididymis undergo functional decline, impacting motility and fertilization capacity.

Purpose of the Study:

  • To investigate nuclear and chromatin changes in spermatozoa residing in extra-epididymal sites.
  • To assess DNA and chromatin integrity in spermatozoa from vasectomized men compared to controls.

Main Methods:

  • Utilized toluidine blue staining to evaluate sperm DNA protamination and chromatin condensation.
  • Employed the sperm chromatin structure assay (SCSA) with flow cytometry to measure DNA damage and sensitivity to acid-induced denaturation.

Related Experiment Videos

Main Results:

  • Spermatozoa from vasectomized patients exhibited significantly higher abnormal protamine disulphide crosslinking and chromatin condensation (p < 0.01).
  • Spermatozoa from vasectomized donors showed significantly greater sensitivity to acid-induced denaturation compared to normozoospermic donors (p < 0.05).

Conclusions:

  • Spermatozoa surviving in extra-epididymal locations are prone to DNA and chromatin abnormalities.
  • These findings contribute to understanding the reduced viability and fertilizing capacity of extra-epididymal spermatozoa.