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The frequency of phospholipase A2 binding of basophilic granulocytes does not decrease during bee-venom-specific

J Irsch1, C König, A Löhndorf

  • 1Department of Genetics, University of Cologne, Germany.

Allergy
|August 12, 1999
PubMed
Summary
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Phospholipase A2 (PLA2)-binding cells are significantly elevated in bee venom-allergic patients, particularly basophilic granulocytes. This finding offers a new method for analyzing effector cells in immediate-type hypersensitivity.

Area of Science:

  • Immunology
  • Allergy Research
  • Cell Biology

Background:

  • Phospholipase A2 (PLA2) is the primary allergen in bee venom.
  • Identifying and quantifying allergen-specific cells is crucial for understanding allergic reactions.

Purpose of the Study:

  • To develop a method for analyzing and enriching PLA2-binding cells from peripheral blood.
  • To investigate the frequency and characteristics of PLA2-binding cells in bee venom-allergic patients.

Main Methods:

  • Utilized high gradient magnetic cell sorting to isolate PLA2-binding cells.
  • Employed flow cytometry to determine the frequency of PLA2-binding cells in peripheral blood mononuclear cells (PBMC).

Main Results:

  • PLA2-binding cells were found at frequencies up to 2.3% in allergic patients, compared to <0.1% in normal donors.

Related Experiment Videos

  • In severe cases, PLA2-binding cells were predominantly basophilic granulocytes (up to 95%).
  • High numbers of circulating allergen-binding basophils correlated with severe anaphylaxis and immunotherapy complications.

    • Conclusions:

    • Specific enrichment of PLA2-binding cells offers a novel tool for analyzing effector cells in bee venom allergy.
    • This method aids in monitoring immediate-type hypersensitivity in allergic individuals.