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Nitroxide stable radical suppresses autoimmune uveitis in rats
1Department of Ophthalmology, Hadassah-Hebrew University Medical School, Jerusalem, Israel. zami@md2.huji.ac.il
Free Radical Biology & Medicine
|August 12, 1999
Summary
The nitroxide TPL effectively reduced the severity of experimental autoimmune uveoretinitis (EAU) in rats. This antioxidant treatment lowered clinical, biochemical, and histopathological signs of this inflammatory eye disease.
Area of Science:
- Ophthalmology
- Immunology
- Pharmacology
Background:
- Free radicals contribute to experimental autoimmune uveoretinitis (EAU) pathogenesis.
- Nitroxides, like TPL, possess superoxide dismutase-mimicking activity and anti-inflammatory effects.
- TPL's potential to suppress EAU was investigated.
Purpose of the Study:
- To evaluate the efficacy of the nitroxide TPL in suppressing S-Ag induced EAU in Lewis rats.
- To assess TPL's impact on clinical, biochemical, and histopathological markers of EAU.
Main Methods:
- Lewis rats were immunized to induce EAU and treated daily with varying doses of TPL or saline.
- Clinical scores, aqueous humor protein, and retinal lipid peroxidation products were measured.
- Histopathological analysis of ocular tissues was performed.
Main Results:
- TPL readily penetrated the aqueous humor.
- TPL treatment significantly reduced clinical and histological EAU scores compared to controls.
- Aqueous humor protein and retinal lipid peroxidation products were significantly lower in TPL-treated rats, with greater effect at lower doses.
Conclusions:
- TPL demonstrates significant therapeutic potential in reducing the severity of S-Ag induced EAU.
- The anti-inflammatory effect of TPL is likely mediated by superoxide radical scavenging.
- Further investigation into TPL's mechanisms in EAU is warranted.
