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Repeated peripheral stem cell mobilization in healthy donors: time-dependent changes in mobilization efficiency
A Tichelli1, J Passweg, T Hoffmann
1Haematological Laboratory, Department of Central Laboratory, University Hospitals, Basle, Switzerland. tichelli@datacomm.ch
British Journal of Haematology
|August 12, 1999
Summary
Repeated mobilization of peripheral blood stem cells using G-CSF yields fewer CD34+ cells with shorter intervals. Adequate stem cells can still be collected within two months for transplantation.
Area of Science:
- Hematology
- Transplantation Immunology
Background:
- Peripheral blood stem cell (PBSC) mobilization is crucial for allogeneic transplantation.
- Recombinant granulocyte-colony stimulating factor (G-CSF) is a standard agent for PBSC mobilization.
Purpose of the Study:
- To evaluate the efficiency of repeated PBSC mobilization in healthy donors.
- To determine the impact of the time interval between mobilizations on stem cell yield.
Main Methods:
- Analysis of 10 healthy donors undergoing two PBSC mobilization procedures.
- Administration of G-CSF (10 microg/kg/d) for both mobilizations, starting collection on day 5.
- Quantification of leucocyte and CD34+ cell counts in peripheral blood and apheresis products.
Main Results:
- A significant decrease in CD34+ cell counts was observed in peripheral blood (median 81.2 vs 50.4 x 10(6)/l) and apheresis products (319.8 vs 275.7 x 10(6)) during the second mobilization.
- Reduced stem cell yield correlated with shorter time intervals between the two mobilization procedures (r2=0.51 for peripheral blood, r2=0.74 for apheresis).
Conclusions:
- Repeated PBSC mobilization within a short timeframe reduces stem cell yield in healthy donors.
- Adequate stem cell numbers can be repeatedly mobilized, but sufficient time between procedures is recommended for optimal collection.