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Related Experiment Videos

Human osteoclasts derive from CD14-positive monocytes.

H M Massey1, A M Flanagan

  • 1Department of Histopathology, Imperial College of Science, Technology and Medicine at St Mary's, London.

British Journal of Haematology
|August 12, 1999
PubMed
Summary

Osteoclasts, bone-resorbing cells, largely originate from CD14-positive monocytes. This research identifies the key precursor cells involved in osteoclast differentiation and bone resorption.

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Area of Science:

  • Cell Biology
  • Immunology
  • Bone Biology

Background:

  • Osteoclasts are crucial for bone remodeling, responsible for bone resorption.
  • Their precise cellular origin and precursor phenotype have been a subject of investigation.

Purpose of the Study:

  • To identify the specific phenotype of the osteoclast precursor cell.
  • To elucidate the cellular source of osteoclasts.

Main Methods:

  • Osteoclasts were generated in vitro by co-culturing peripheral blood mononuclear cells (PBMNCs) with the UMR 106 cell line.
  • Immunophenotyping was performed using markers such as calcitonin receptor (CTR), CD14, CD11b, and CD11c.
  • The role of CD14-positive monocytes in osteoclastogenesis was assessed by comparing bone resorption activity.

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Main Results:

  • Initially, osteoclast precursors (CTR-positive cells) co-expressed monocyte markers (CD14, CD11b, CD11c).
  • With differentiation, expression of these monocyte markers decreased significantly.
  • Enrichment of CD14-positive monocytes enhanced osteoclast bone resorption activity.

Conclusions:

  • Osteoclasts predominantly differentiate from CD14-positive monocytes.
  • This finding clarifies the cellular lineage of osteoclasts and their role in bone resorption.